Have you ever wondered what’s happening in the DNA profiling labs? You would have to spend many devoted hours reading a proper textbook if you need in depth knowledge. However, I can provide you with some ideas using a series of excerpts from my thriller, The Sex Tourist. This first excerpt is from Book Two, Chapter 7 – Polymerase Chain Reaction:
Inside the PCR box, the heat is ripping apart millions of DNA strands like a myriad of disappointed punters tear up their losing lottery tickets after a draw on Saturday night in front of their TV set. The ten-minute polymerase activation period is over, and the mixture inside keeps cooling down – which doubles the amount of DNA fragments – and heating up a few seconds later, just to tear all of them apart again.
I think about the “Wheat on the Chessboard” story. The king was so pleased to learn chess, a newly invented game at the time, that he asked the inventor of the game what he wanted for reward. The inventor asked for some wheat grains. He wanted one grain of wheat on the first square of a chessboard, two grains of wheat on the second square, four grains on the third square, eight grains on the fourth square, and so on, doubling the number of grains of wheat with each square up to the last square. The king thought this was very easy until he realized that all the grains in the world would not be enough to grant this reward.
It takes less than two hours for this PCR box to grant me what the king could not. The only difference is that it’s providing me with billions of amplified DNA fragments instead of wheat grains.
The cocktail contains minuscule amounts of very special liquids supplied by various overseas companies. My thesis depends on the results of this experiment and the next. Mr. Robert Szabo, my thesis supervisor, is standing next to me as I’m preparing more components and DNA samples from infectious bacteria found in sheep for the next round of experiment. I’m using a DNA Mini kit ordered from a German biotech company.
The same company could supply me with the kit I need for my own private DNA samples. That would only be the first step for getting the DNA profile of Fitzgerald. My hope is that certain segments of his minuscule amount of DNA on the swabs are going to be copied millions of times with the help of this PCR box, to provide me with his distinct genetic pattern. I’ve read many books and spent countless hours on the internet investigating what’s needed for purifying, quantifying, amplifying, and sequencing my human DNA samples.